Fig. 7

Exposure to myelin and dead cellular debris stimulates CAM marker Dectin1/Clec7a in microglial culture. a Using purified, serum-free, microglial cultures, proinflammatory cytokines and immune stimulants failed to increase the proportion of microglia expressing Dectin1. b, c Quantification and representative immunocytochemical image demonstrating myelin debris exposure increases the proportion of Dectin1 expressing microglia, in purified, serum-free, cultures. d Schematic illustrating experimental procedure to kill cultured oligodendrocytes with LPC and subsequent chelation of LPC with albumin to halt toxicity. e, f Quantification and representative immunocytochemical image demonstrating that exposure to LPC-treated oligodendrocyte carcasses stimulates microglial Dectin1. a, b, e n = 3 wells in 2–3 independent experiments (a, e) One-way ANOVA with Dunnett’s multiple comparison test. b Unpaired T-Test. Error bar ± SEM. Scale bar, (c, f) 50 μm