Fig. 7

Nup62 and KPNB1 colocalize with pathological pTDP-43 aggregates in human post-mortem brain and spinal cord tissue. Co-immunofluorescence (IF) staining was conducted in fixed spinal cord, posterior hippocampus, and motor cortex of neuropathologically diagnosed ALS and FTLD cases, respectively, and including unaffected control tissue. a,b High resolution co-IF staining of pTDP-43 (red) and Nup62 or KPNB1 (green) with Hoechst (blue) in spinal cord (ALS) and hippocampus (FTLD). Each image is shown as a panel of projected optical sections from each z-series for red and green channels and merged with Hoechst DNA staining. Volume rendered z-series of all channels are shown as the 3D inset. Scale bar: 5 μm. c,d Hippocampal tile scan images of Nup62 or KPNB1 co-IF with pTDP-43 aggregates in the sFTLD-B#1 case. Scale bar: 50 μm. e,f High resolution co-IF staining of pTDP-43 (red) and Nup62 or KPNB1 (green) with Hoechst (blue) in the motor cortex. Scale bar: 5 μm. g,h Cortical tile scan images of Nup62 or KPNB1 co-IF with pTDP-43 aggregates in TARDBP ALS case. Scale bar: 50 μm