Fig. 6

A LADAM subcluster is observed in white-matter-associated microglia. A UMAP plot of 12-month-old ‘LADAM’. B Bar plot showing the distribution of 4 genotypes across 4 LADAM clusters. C UMAP plot of LADAM, showing a small cluster (C2, purple-colored cells) that resemble WAM gene modules [25]. D UMAP plots showing gene expressions that are highly enriched genes, including WAM genes (Vim and Lgals3) in the LADAM C2 cluster (left), and heatmap showing expression of WAM gene modules in C2 across genotypes (right). E Iba1 and Siglec-G immunostaining (left) and quantification (right) in 6-month-old (top) and 12-month-old (bottom) corpus callosum in WT, APP;PS1, Tau4RΔK, and Tau4RΔK-AP. F Siglec-10 immunostaining in human cortex in control, Braak stage 6, and nAD tauopathy (numbers on figure panels indicate BRC# In Table S11, immunostaining of other Braak stages are shown in Fig S15), with bar graphs showing quantification of Siglec-10 positive cells in gray (top) and white (bottom) matter. G Schematic showing human snRNA-Seq on Stage 4 and 6 SFG (left) and UMAP plot showing LADAM C2 enriched gene module (right). H UMAP plots showing expression of LADAM C2 cluster markers in human snRNA-Seq. Red boxes show high magnification views. CC = Corpus Callosum. Scale bars = 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001