Fig. 3

Cleavage of gp130 by BACE1 in vitro. a Scheme illustrating domain structure of gp130 and its proteolytic cleavage by BACE1, leading to sgp130 secretion. The Ig-like domain (blue triangle) and the five fibronectin type III domains (orange and blue) in the gp130 ectodomain are indicated. b In vitro cleavage assay of purified gp130 with BACE1 in the presence or absence of the BACE inhibitor C3. Western blot detects HA tag at the N-terminus of gp130 or the HIS tag at the C-terminus of gp130 and the His-tag of BACE1. For the lowest panel an antibody to the natural C-terminus of gp130 was used. Shown are representative blots for N = 3 independent experiments