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Table 1 Primary and secondary antibodies used for IHC

From: Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier

Primary antibody or lectin (manufacturer, catalog #, dilution used)

Secondary antibody (manufacturer, catalog #, dilution used)

Human amyloid-β

 Rabbit anti-human β-amyloid

(Cell Signaling, 8243S, 1:500)

Alexa fluor 647-conjugated donkey anti-rabbit (Invitrogen, A-31573,1:500)

Smooth muscle cells

 Mouse FITC conjugated α-smooth muscle actin (SMA) (Sigma, clone 1A4, F3777, 1:500)

N/A

Vasculature

 Dylight 488-conjugated L. esculentum lectin (Vector Labs, DL-1174,1:200)

N/A

Fibrinogen

 Rabbit anti-human fibrinogen, cross-reacts with mouse fibrinogen [48] (Dako, A0080,1:400)

Alexa fluor 568-conjugated donkey anti-rabbit (Invitrogen, A-10042,1:500)

PICALM

 Goat anti-human PICALM, cross-reacts with mouse PICALM (Santa Cruz, sc-6433, 1:200)

Alexa fluor 568-conjugated donkey anti-goat (Invitrogen, A-11057,1:500)

Microglia

 Rabbit anti-mouse Iba1

(Wako, 019-19741; 1:500)

Alexa Fluor 568–conjugated donkey anti-rabbit (Invitrogen, A-10042, 1:500)

Astrocytes

 Rabbit anti-bovine GFAP, cross-reacts with mouse GFAP (Dako, Z0334, 1:500)

Alexa Fluor 568–conjugated donkey anti-rabbit (Invitrogen, A-10042, 1:500)

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Molecular Neurodegeneration

ISSN: 1750-1326