Fig. 5
From: Carriers of the p.P522R variant in PLCγ2 have a slightly more responsive immune system
Detection of phagocytosis and ROS production in monocyte subsets after stimulation with pHRodo™ Green E. coli bioparticles. A To evaluate the outcome of the phagocytosis assays, three different readouts were used per population; % of cells that were phagocytosing, the average amount of particles phagocytosed per cell, and the ROS production upon phagocytosis (as measured by conversion of DHR123 into R123). The average amount of particles phagocytosed per cell and the ROS production were further combined into one value; the ROS produced per particle, or the ‘normalized ROS’ (calculations are indicated in the Methods). The values are presented for the CD62L- classical monocyte (cMo) subset B The normalized ROS of all four defined non-classical monocyte (ncMo) subsets, based on expression of CD36 and SLAN. Differences were evaluated by means of Mann-Whitney U test. All readouts were corrected for background/baseline activation by subtracting the value of the control (ice) from the activated (37 °C) sample. * p < 0.05. C median normalized ROS production in neutrophils and all monocyte subsets. % increase in normalized ROS in carriers vs non-carriers is indicated behind each population in the legend