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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: BACE1 regulates expression of Clusterin in astrocytes for enhancing clearance of β-amyloid peptides

Fig. 5

Clusterin underlies astrocyte endocytosis of Aβ in vitro. A Western blot of Bace1-null and WT-primary astrocytes pretreated with either 80 pmol of Clu siRNA or 80 pmol of control scrambled siRNA and incubated with 2 μM of Aβ42 for indicated times. Images indicate major bands for Aβ42 (human, oligomeric) and GAPDH. B Quantification of Western blot Aβ42 band intensity normalized to actin (N = 3, * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001; One-way ANOVA with Tukey post testing comparing between samples. C Confocal imaging of astrocyte primary cultures from BACE1-null and WT and pretreated with either Clu or control scrambled siRNA then treated with 2 μM of Aβ42 tagged with fluorescent Hilyte-555 for indicated incubation times. Stained with phalloidin (green) to mark F-actin and ToPro3 (blue) to mark nuclei. Scale bars represents 10 μm

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