Fig. 5
HDL reduces α-syn aggregation more efficiently than HSA. A ThT protein aggregation assay performed using 0.7 mg/mL of recombinant α-syn in PBS pH 7.4 in the presence of different concentrations of HSA (0, 0.3, 6.7 and 43 mg/mL) and HDL (0, 0.12 and 0.57 mg/mL). To remove the background fluorescence, the average fluorescence of three replicates containing the same amount of HSA and HDL without α-syn was subtracted prior to the analysis. The data represent the average fluorescence of three replicates with error bars representing the SEM. B Fmax and fitted A2 parameters fitted from individual traces and averaged on the three replicates are shown. C Inverted and window/level-adjusted image of the dot-blot assay performed on the final reaction products of HSA and HDL-containing samples. Dot-blots were probed with OC (detection of fibrils) and A11 (detection of amorphous oligomers) conformational antibodies. D Adjusted (background subtracted) integrated density measured in a circular region of interest (0.35 cm2) surrounding dots relative to samples 1–5 with error bars representing the standard deviation of the background noise. The average grey level is significantly lower (p < 0.001) for samples with HDL with respect to all the HSA concentrations tested by applying two-tailed Student’s t-test both for OC and A11 antibodies