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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Mutations in α-synuclein, TDP-43 and tau prolong protein half-life through diminished degradation by lysosomal proteases

Fig. 1

Lysosomal proteases digest α-syn, TDP-43 and tau in a selective fashion. Recombinant, human, full-length α-syn, TDP-43 or 2N4R tau (1 µg) were incubated with 1 µM lysosomal protease for 1 h at the indicated pH. Reactions were subjected to SDS-PAGE and gels visualized by silver stain. Gels are representative of n = 3 independent replicates. Protein and protease combinations are organized according to those in which the protease can cleave (a) or cannot cleave (b) the protein. Full-length gels are shown in Supplementary Data 1 and control reactions for those proteases that cannot cleave any of the substrates are shown in Figure S1

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Molecular Neurodegeneration

ISSN: 1750-1326