Alzheimer-specific variants in the 3'UTR of Amyloid precursor protein affect microRNA function

Background APP expression misregulation can cause genetic Alzheimer's disease (AD). Recent evidences support the hypothesis that polymorphisms located in microRNA (miRNA) target sites could influence the risk of developing neurodegenerative disorders such as Parkinson's disease (PD) and frontotemporal dementia. Recently, a number of single nucleotide polymorphisms (SNPs) located in the 3'UTR of APP have been found in AD patients with family history of dementia. Because miRNAs have previously been implicated in APP expression regulation, we set out to determine whether these polymorphisms could affect miRNA function and therefore APP levels. Results Bioinformatics analysis identified twelve putative miRNA bindings sites located in or near the APP 3'UTR variants T117C, A454G and A833C. Among those candidates, seven miRNAs, including miR-20a, miR-17, miR-147, miR-655, miR-323-3p, miR-644, and miR-153 could regulate APP expression in vitro and under physiological conditions in cells. Using luciferase-based assays, we could show that the T117C variant inhibited miR-147 binding, whereas the A454G variant increased miR-20a binding, consequently having opposite effects on APP expression. Conclusions Taken together, our results provide proof-of-principle that APP 3'UTR polymorphisms could affect AD risk through modulation of APP expression regulation, and set the stage for further association studies in genetic and sporadic AD.


Findings
AD is the most common form of dementia worldwide. Pathologically, the disease is defined by the intracellular accumulation of aggregated and hyperphosphorylated protein tau and the extracellular deposition of Aβ peptides, derived by proteolytic processing of APP. In genetic AD, mutations in the genes coding for APP, PSEN1 and PSEN2 lead to APP processing dysregulation resulting in Aβ over-production, accumulation and deposition, which ultimately leads to neuronal death [1]. Accumulating evidences also support the notion that increasing APP protein levels directly results in Aβ over-production [2], and that APP overexpression alone is sufficient to induce neurodegeneration an dementia [3][4][5][6].
miRNAs function as negative regulators of gene expression regulation, and play a critical role in neuronal function and survival [7]. These small (~21nt) non-coding RNAs interact with the 3'UTR of their target messenger RNA (mRNA) transcripts by partial sequence complementarity resulting in mRNA destabilization and/or translational inhibition [8,9]. This function is dependent on the miRNA seed region, comprising nucleotides 2-8 of the mature miRNA sequence. As changes in APP expression is intimately involved in AD development, several groups have now investigated the impact of miRNA modulation on APP expression. These studies identified a number of miRNAs capable of regulating APP expression in vitro and in vivo, including miR-20a, miR-17 (previously referred as miR-17-5p, http://www.mirbase.org), miR-106a, miR-106b, miR-101 and miR-16 [10][11][12][13][14][15]. Interestingly, miR-101, and miR-106b have been shown to be down-regulated in AD brain, therefore potentially contributing to increased APP expression and Aβ production [16,17].
Increasing evidence supports the hypothesis that genetic variants that either abolish existing miRNA binding sites or create illegitimate miRNA binding sites could contribute significantly to risk for neurodegenerative disorders. For instance, Wang et al. showed that a SNP located in the 3'UTR of the fibroblast growth factor 20 (FGF20) gene confers risk for developing PD, possibly by loss of miR-433 binding [18]. In addition, Rademakers et al. showed that increased binding of miR-659 to the 3'UTR of the progranulin (GRN) gene provides an important risk for TDP43-positive frontotemporal dementia [19]. More recently, Bettens et al. identified a number of AD-specific genetic mutations in the 3'UTRs of APP and BACE1 [20]. We extended these findings and established a detailed list of miRNAs with potential binding sites in or near polymorphisms located in the 3'UTR of human APP (hAPP) ( Table 1). These include the APP variants T171C (unknown SNP ID), A454G (unknown SNP ID) and A833C (SNP ID rs3200120). These bioinformatics predictions were performed using logarithms available on-line, such as Microcosm [21], TargetScan [22] and microRNA. org [23]. In this study, we focused on polymorphisms predicted to abrogate (completely or partially) miRNA binding.
In order to validate these observations in more physiological conditions, we transfected our candidate pre-miRs into mouse neuroblastoma Neuro2A cells, a model previously used to study neuronal APP expression regulation by miRNAs [10]. Except for miR-147, each miRNA seed region was conserved between human and mouse APP 3'UTRs [22]. For this reason, miR-147 was omitted from the mouse cell line experiments. In Neuro2A cells, all pre-miRs tested decreased endogenous APP expression when compared to the scrambled miRNA control ( Figure 1C). We also transfected human HeLa cells with our candidate pre-miRs. All but one miRNA (miR-655) decreased endogenous APP expression when compared to the scrambled miRNA control ( Figure 1D). Notably, miR-147 could efficiently down-regulate endogenous APP in these cells. Taken together, these data add to the The SNP ID, the nature of the polymorphism, as well as the patient specificity are indicated. The unknown SNP IDs can be found in Bettens et al. [20]. miRNAs which are predicted to interact with the 3'UTR of hAPP are listed, and it is noted when the SNP is located within the seed-region of the miRNA in question. Y = yes, N = no.
growing list of miRNAs that could regulate endogenous APP expression in cultured cells, including neuronal-like cells. These include miR-147, miR-323-3p, miR-644 and miR-153, in addition to the previously identified miR-20a and miR-17. We next tested whether the "APP-positive" miRNAs were affected by the APP 3'UTR polymorphisms (Table 1). For these experiments, we focused on AD-specific SNPs, and generated hAPP 3'UTR luciferase constructs with T171C or A454G mutations. Our screens indicated that miR-147, but not miR-644 or miR-323-3p, was significantly affected by the T171C mutation when compared to the wild-type (WT) construct ( Figure 2B, upper panel). In a similar set of experiments, we could show that miR-20a, but not miR-153 or miR-17, was affected by the A454G mutation ( Figure 2B, lower panel). As expected, miR-147 is a less potent inhibitor of APP expression in the presence of T171C. On the other hand, and surprisingly, miR-20a further decreased the expression of APP in the presence of A454G. For miR-147, the effect may be explained by the fact that T171C is located immediately adjacent to the miR-147 seed region, therefore directly inhibiting miRNA binding ( Figure 2C, upper panel). This effect can also be explained as the mutation increased the binding energy (ΔG = -18.9 kCal/mol vs. -16.8 kCal/mol) between miR-147 and the 3'UTR of hAPP, which makes the binding less Figure 1 In vitro functional analysis of APP A. Schematic representation (not to scale) of the luciferase reporter construct used in this study. TK; thymidine kinase promoter, AAAA(n); PolyA site B. HEK293T cells were transfected with 50nM pre-miRs (as indicated) as well as a reporter construct containing the 3'UTR of hAPP. The cells were lysed 24h post-transfection and luciferase signal was measured. Signals were normalization for transfection efficiency and graph represents the luciferase signals compared to the scrambled control (SCR). Statistical significance was assessed by Student paired t-test. (* = p < 0.05, ** = p < 0.01, *** = p < 0.001) C. and D. Neuro2A cells (C) and HeLa cells (D) were transfected with 50nM pre-miRNAs (as indicated). The cells were lysed 48h post-transfection and western blotting was performed. Representative (n = 3, in triplicate) western blots are shown. The ratios of the APP/β-Actin signals are presented. Measurements were normalized to the scrambled control (SCR). Statistical significance was assessed by Student's paired t-test. (* = p < 0.05, ** = p < 0.01, *** = p < 0.001). favorable. Although we observe a net gain of function of miR-20a towards the SNP A454G form compared to the WT 3'UTR, A454G is not predicted to change the binding energy (ΔG = -22.8 kCal/mol) between miR-20a and the 3'UTR of hAPP, and the SNP is also not located within the seed region ( Figure 2C, lower panel). Taken together, we identified two miRNAs, that is, miR-147 and miR-20a, affected by AD-specific 3'UTR SNPs.
In conclusion, we provide evidence for the first time that polymorphisms located in the 3'UTR of hAPP may affect its expression, at least in the experimental conditions tested here. Indeed, we show that two AD-specific 3'UTR variants previously identified by Bettens and colleagues [20] affect the modulating activity of miR-147 and miR-20a on the expression of APP. SNP T171C decreases the ability of miR-147 to down-regulate APP, theoretically leading to increased APP and Aβ production. On the other hand, SNP A454G increases the effect of miR-20a, suggesting that APP expression is reduced in these patients. Although these data seem to contradict with the main hypothesis that increased APP levels lead to AD, some reports indicate that decreasing the APP levels might have deleterious consequences in the brain [24,25]. Another possibility is that miR-20a levels (or function) vary depending on brain region or disease state, therefore only locally affecting APP. In line with this hypothesis, our preliminary data suggest that certain "APP-positive" miR-NAs are differently expressed between human regions (Delay et al., not shown). Finally, we cannot exclude at this stage of investigation that the second, less functional miR-20a binding site located at position 709-715 in the hAPP 3'UTR [10], could become more prone to miRNA regulation in the presence of SNP A454G. While followup studies are required to evaluate the incidence of these variants in other populations, our results suggest that 3'UTR mutations may contribute to risk for AD development. These studies also set the stage for validation studies regarding APP expression regulation by specific miRNAs in vivo in the brain, and further evaluation of 3'UTR variants in AD-related genes in general.

Cell culture
Human HEK293 and HeLa cells, as well as mouse Neuro2A cells, were cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal bovine serum. One day before transfection, HEK293 cells were plated at 100,000 cells per well in 24-well plates, Neuro2A cells at 192,000 cells per well in 6-well plates, while HeLa cells were plated at a 20% confluence in 6-well plates. Transfection was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions.

cDNA constructs
The full-length hAPP 3'UTR luciferase construct was described previously [10]. Mutagenesis was performed by TOPgene technologies (Montreal, Quebec, Canada) and validated by sequencing.

Statistics
Statistical significance of western blots and luminescence quantifications were determined using 1-way ANOVA, 2-way ANOVA or Student's paired t-test as indicated in the text. Calculations were made using the GraphPad Prism 5 software.