Figure 2

Effects of Dimebon on glutamate-induced Ca²⁺signals. (A-B), Repetitive application of 20 μM glutamate induces Ca²⁺ signals in MSN from the WT (A) and YAC128 (B) mice. (C-D), The same experiment as in (A) and (B) was performed in the presence of 10 μM Dimebon with WT (C) and YAC128 (D) MSN. (E-F), The same experiment as in (A) and (B) was performed in the presence of 50 μM Dimebon with WT (E) and YAC128 (F) MSN. The traces shown on panels (A-F) are average traces from all MSN for each experimental group. (G) The average increase of basal Ca²⁺ level (mean ± SE, n is the number of MSN analyzed) after 20 pulses of glutamate are shown for WT MSN (n = 16), YAC128 MSN (n = 21), WT MSN in the presence of 10 μM Dimebon (n = 44), YAC128 MSN in the presence of 10 μM Dimebon (n = 41), WT MSN in the presence of 50 μM Dimebon (n = 17) and YAC128 MSN in the presence of 50 μM Dimebon (n = 44).