Figure 6From: A novel brain-enriched E3 ubiquitin ligase RNF182 is up regulated in the brains of Alzheimer's patients and targets ATP6V0C for degradationRNF182 exhibits ubiquitin E3 activity. His-tagged recombinant RNF182 protein was incubated with or without E1, E2 or ubiquitin, and ubiquitination patterns were detected using an anti-ubiquitin antibody. The GST-SIAH-1 protein was used as a positive control.Back to article page