Figure 5

Wnt-5a prevents the changes induced by Aβ oligomers on PSD-95 clustering and in synaptic contact. (A) Electron micrograph of the material corresponding to Aβ-oligomers taken in Phillips Tecnai 12 EM. The picture shows the amylospheroids. Scale bar = 100 nm. (B) Representative immunoblot of total PSD-95 levels of neurons exposed to 0.05 or 0.5 μM Aβ oligomers in the presence or absence of Wnt-5a and a quantification of densitometry are shown (n = 3). *P < 0.05. (C), Representative neurite images of double immunofluorescence of PSD-95 (green) and synapsin-1 (red) and stained with phalloidin (blue) from samples subjected for 1 h to control, Aβ, Aβ/Wnt-5a and Aβ/Wnt-5a/sFRP treatments. Merged images show the apposition of the pre-synaptic (red) and post-synaptic (green) boutons. (D), Quantification of synaptic PSD-95 over total synapsin-1 and; (F), quantification of synaptic PSD-95 over total PSD-95 upon the different treatments used in C (n = 4). Bar represents the mean ± SEM (*p < 0.05 Student's t test).