Figure 1From: Isoform of APOE with retained intron 3; quantitation and identification of an associated single nucleotide polymorphismPCR Detection of APOE-NL and APOE-I3. PCR primer positions are depicted relative to the four exons of APOE (A). Two SNPs are also shown: rs12982192 within intron 3 and rs429358, which determines APOE4 status, in exon 4. PCR of 25 or 28-33 cycles was used to detect APOE-NL and APOE-I3, respectively, in pooled brain cDNA samples (B).Back to article page