Figure 6
Reduction of Aβ 42 levels decreases TDP-43 and TDP-35 levels. (A-B) Representative microphotographs depicting CA1 pyramidal neurons from 6-month-old 3×Tg-AD and APP/tau mice stained with an anti-Aβ antibody (n = 6/group). (C) Representative microphotograph depicting CA1 pyramidal neurons from APP/tau mice stained with the anti-oligomeric antibody M17/3. (D) Representative Western blots of protein extracted from the brains of 6-month-old NonTg, APP/tau and 3×Tg-AD mice (low salt fraction), probed with a polyclonal anti-TDP-43 antibody. β-Actin is used as a loading control. A longer exposure time was necessary to see the less abundant low molecular weight fragments. (E-F) Quantitative analysis of the blots (n = 6/genotype) shows that in APP/tau mice, the steady-state levels of TDP-43 and TDP-35 are significantly lower in APP/tau mice compared to 3×Tg-AD mice (p < 0.05). The levels of TDP-43 and TDP-35 were not statistically significant different between APP/tau and NonTg mice. Data are presented as means ± SEM and were analyzed using one-way ANOVA following by post hoc Bonferroni test to determine individual differences in groups. Abbreviation: exp = exposure.