Figure 6
Three-dimensional reconstruction of cofilin-stained rods in deconvolved confocal image stacks from organotypic slices. Treatment of organotypic slices with Aβd/t results in a profound increase in cofilin-immunostained rods in the dentate gyrus/mossy fiber tract (DG/MFT) and a global change in cofilin distribution in cells in this region. (A) Single focal plane of non-rod-forming region near the CA3 compared to a rod hot spot in the dentate gyrus. Rods are evident in this single plane. (B) Three dimensional stack of planes from a cofilin stained control and Aβd/t-treated slice. Deconvolution of the confocal image stacks and thresholding the image by removing the lowest 20% of signal (lower panels) provides striking evidence of rod formation in this region. Hundreds of rods can be observed, which contain virtually all of the remaining immunostained cofilin.