Figure 2From: Rheumatoid arthritis-associated polymorphisms are not protective against Alzheimer's diseaseBACE2 isoforms present in human brain. Human brain cDNA samples were screened for BACE2 isoforms using conventional PCR amplification across the alternatively spliced regions of exons 7 and 8. (A) Four alternatively spliced BACE2 isoforms were detected among multiple individuals. (B) Direct sequencing of the resulting splice variants confirmed their identities as BACE2d7, BACE2d8 and BACE2d7/8. Deletion of BACE2 exon 7 results in an in-frame deletion of 50 amino acids whereas deletions of BACE2 exon 8 or exons 7 and 8 lead to a frameshift and premature truncation of the protein.Back to article page