Figure 4
13C 6 -leucine brain apoE labeling in the presence of ABCA1 overexpression and deletion. (A) Cohorts of wildtype and ABCA1 transgenic mice and (C) wildtype and ABCA1−/− mice were labeled with 13 C6-leucine and the brains isolated after predetermined time points. Note that separate groups of animals were used as the wildtype controls for the ABCA1 Tg and ABCA1−/− mice. The apoE labeled/unlabeled ratios were then calculated and the data plotted as in Figure 3. For FCR measurements, the monoexponential slopes were measured for (B) ABCA1 Tg and (D) ABCA1−/− mice and their respective Wt controls (n = 5-6 mice per time point, error bars represent SEM, dotted lines represent 95% confidence band).