Figure 3

TH-immunostaining and fragmentation of Golgi apparatus in I2020T LRRK2 TG mice. (A) TH staining of DA neurons in the substantia nigra compacta (SNc). A series of midbrain sections from I2020T LRRK2 TG and NTG littermate mice of 18 months were stained with antibody against TH. Higher-magnification views of the boxed area show the morphology of TH-immunoreactive neurons. Scale bar: 50 μm. Right panel shows quantification of TH-immunoreactive neurons in the SNc by stereological counting. 10 weeks (NTG, n=3; TG, n=3), 18 months (NTG, n=3; TG, n=3). (B) TH staining of DA neurites in the dorsal striatum of TG and NTG littermate mice of 18 months. Right panel shows optical density analysis. 10 weeks (NTG, n=3; TG, n=3), 18 months (NTG, n=3; TG, n=3). (C) Immunostaining of TH and GM130 (cis-Golgi) in the SNc. TH-positive neurons were classified into three groups – normal, partial fragmentation, and severe fragmentation – depending on the degree of fragmentation of Golgi structures (50 neurons were counted for each mouse; NTG, n=3; TG, n=3; 10 weeks). Scale bar: 10 μm. Quantitative data are expressed as mean ± SEM and were analyzed by Student’s t test; *p<0.05.