Figure 7
From: Regulation of mitochondrial permeability transition pore by PINK1
Normal levels of oxidative stress markers and normal production of reactive oxygen species in PINK1 −/− MEFs. A. Left panel: OxyBlot analysis of mitochondrial fractions showing levels of protein carbonyls in both genotypes. Right panel: Quantification of levels of protein carbonyls in PINK1−/− and +/+ MEFs. B. Levels of lipid peroxidation measured by the TBARS assay in PINK1−/− and +/+ MEFs. C. Kinetics of H2O2 production in isolated mitochondria measured by following Amplex Red fluorescence over time. D. Kinetics of cytosolic superoxide anion O2- production measured in resuspended MEFs by following DHEt fluorescence over time. E. Representative flow cytometry dot plots showing calcein fluorescence in PINK1−/− and +/+ MEFs in the presence of Co2+ after antioxidant treatment with Tocopherol (50 μM, 4 hr) or NAC (1 mM, 2 hr). F. Quantification of calcein fluorescence in PINK1−/− and +/+ MEFs following treatment with Tocopherol (50 μM, 4 hr) or NAC (1 mM, 2 hr). All data are expressed as mean ± SEM. *p < 0.05.