Figure 10

Different receptors and S100B or Aβ1–42 co-localisation in transfected HEK293 cells. Untransfected or FPR1-, FPRL1- or RAGE expressing HEK293 cells were incubated with 2.4 μM S100B or 1 μM Aβ1–42 for 2 h. Cells were fixed and labelled with anti-FPR1, -FPRL1, -RAGE and anti-S100B or anti- Aβ1–42 antibodies. Localisation of FPR1, FPRL1 or RAGE and S100B (A) or Aβ1–42 (B) was examined by double fluorescence microscopy. Bisbenzimide was used for nuclear counter-staining (blue). The figures show representative results from one of three independent experiments, each performed in duplicate. Scale bar: 20 μm. The right columns show the resulting scatter plot of intensities across the two channels. The Pearson correlation coefficient r_p and Spearman correlation coefficient r_s are indicated on the scatter plots. For FPR1 (red channel) and S100B or Aβ1–42 (green channel), poor or low co-localisation; for FPRL1 (red channel) and S100B or Aβ1–42 (green channel), low or good co-localisation and for RAGE (red channel) and S100B or Aβ1–42 (green channel), both low co-localisation.