Progressive morphological changes and impaired retinal function associated with temporal regulation of gene expression after retinal ischemia/reperfusion injury in mice

Table 2 Gene ontology clusters downregulated in the mouse retina at various time points after I/R injury

Time		Ontology	Enrichment score	P value (P < 0.05)
0 day		No significant changes
6hr	CC	extracellular region	1.64	3.10E-02
1 day		No significant changes
3 day	BP	protein-DNA complex assembly	1.63	7.20E-03
	MF	DNA binding	1.63	2.10E-02
	CC	protein-DNA complex	1.63	8.30E-03
7 day		No significant changes
14 day	BP	eye development	1.9	4.70E-02
14 day	MF	structural constituent of eye lens	1.9	1.40E-03
21 day		No significant changes
28 day	BP	G-protein coupled receptor protein signaling pathway	3.16	3.10E-05
28 day	CC	integral to membrane	3.16	5.00E-02

Microarray data were filtered for selection of downregulated genes lower than -1.5 fold (log₂) after normalizing with control eyes. Data were further analyzed using DAVID and clustered according to ontology with 3 categories (BP, MF and CC) at 8 time points (0 hr, 6 hr, 1, 3, 7, 14, 21 and 28 days) after I/R injury.

ISSN: 1750-1326