Figure 2
From: Effects of the C57BL/6 strain background on tauopathy progression in the rTg4510 mouse model
2.5-month-old rTg4510 B6 mice have increased human tau expression compared to rTg4510 mice. (A-B) Western blot analyses of the soluble fractions of whole brain lysates from rTg4510B6 and rTg4510 mice at 2.5 months of age. (A) Representative western blots of soluble human tau (E1 antibody), human and mouse tau (Tau5 antibody), and phosphorylated tau (CP13 and PHF1 antibodies) with a NT littermate shown as a negative control. (B) Densitometric quantification of E1 (human tau), Tau5 (human and mouse tau), CP13 (pS202 tau) and PHF1 (pS396/404 tau) normalized to GAPDH. rTg4510B6 mice had increased soluble human tau, indicating greater tau transgene expression than age-matched rTg4510 mice. No differences in soluble phospho-tau levels were detected using CP13 and PHF1 antibodies. Each dot represents an individual mouse with the mean indicated by the black line. n = 9-10 per cohort. *P < 0.05 (Student’s t-test).