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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Is L-methionine a trigger factor for Alzheimer’s-like neurodegeneration?: Changes in Aβ oligomers, tau phosphorylation, synaptic proteins, Wnt signaling and behavioral impairment in wild-type mice

Fig. 2

Accumulation of Aβ peptides and formation of Aβ oligomers are induced in animals treated chronically with L-methionine. Aβ42 and (a and c) Aβ40 (b and d) were examined by two different ELISA assay. e Histological slices were treated by immunohistochemistry with WO2 antibody to analyze Aβ levels and location. Hippocampus sections of control mice at 20x and 40x; L-methionine sections at 20x and 40x (a and b). The right panel shows the quantification (n ≥ 4) f) Immunoprecipitation of 100 μg of total proteins from the hippocampus with A11 antibody and western blot with 4G8 antibody and c-jun antibody (i). Histological slices were treated by immunofluorescence with 4G8 antibody to analyze Aβ levels and location (ii). Hippocampus sections of control mice at 20x and L-methionine sections at 20x and 40x). Bars are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Neurodegeneration

ISSN: 1750-1326