Fig. 6

The distribution of BIN1 in mouse brain. BIN1 immunolabeling is in green and the marker labeling is in red. Nuclear staining with Hoechst is in blue. a Immunofluorescence labeling of coronal sections with antibodies against BIN1 (N-19 or BSH3) shows overlap with MBP but not with NeuN labeling. b–g Confocal microscopy analysis of BIN1 immunolabeling. b A low magnification image of BIN1 labeling with mAb 2 F11. A higher magnification of the cortex and corpus callosum is shown in the middle and right panels. Thick and thin arrows point to BIN1 labeling of the cell body and barbarized processes in the cortex. c N-19 labeling reveals BIN1-positive cells dispersed throughout the cortical layers. d and e BIN1 and MBP labeling overlap along corticostriatal projections and in the cerebellar white matter. f BIN1 and NeuN labeling are largely distinct in the hippocampus and striatum. BIN1-positive cell bodies (white arrows) and processes in stratum oriens or fiber bundles in alveus (yellow arrows) are indicated. g BIN1 immunolabeling of cell bodies (white arrows) and fiber bundles (yellow arrows) in the striatum does not overlap with neuronal and neuropil labeling of Amphiphysin 1. CC = corpus callosum; CA1 = Cornu Ammonis area 1; SO = stratum oriens; SP = stratum pyramidale