A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein

Table 1 Antibodies (at 1 mg/ml) used for establishing AlphaLISA assays are listed below together with provider information

Clone	Company	Catalog #	Host species	Clonality	Epitope	Specific detection of
Clone	Company	Catalog #	Host species	Clonality	Epitope	Human	Rodent	S129 phos
C-20	Santa Cruz	sc-7011-RL	rabbit	polyclonal	C-terminus	yes	yes	no
LB509	Covance	SIG-39725	mouse	monoclonal	115–122	yes	no	no
asyn204	Cell Signaling	2647BF	mouse	monoclonal	087–111	yes	no	no
asyn211	Abcam	ab80627	mouse	monoclonal	121–125	yes	no	no
4B12	Covance	SIG-39730	mouse	monoclonal	103–108	yes	no	no
pSyn#64	WAKO	014-20281	mouse	monoclonal	124–134	yes	yes	yes
EP1536Y	Abcam	ab173323	rabbit	monoclonal		yes	yes	yes
MJF-R13	Abcam	ab176839	rabbit	monoclonal		yes	yes	yes
11A5	Prothena	N/A	mouse	monoclonal	124–134	yes	yes	yes

ISSN: 1750-1326