Fig. 3From: Deimmunization for gene therapy: host matching of synthetic zinc finger constructs enables long-term mutant Huntingtin repression in miceMicroglial activation in the striatum after various treatments. Representative micrographs of IBA1 immunostained striatal coronal slices, for the control and injected hemispheres, for each treatment at 4 or 6 weeks. ZF-KOX1 samples displayed an apparent increase in Iba1 immunoreactivity in the injected hemispheres, at 4 and 6 weeks after treatment (a, b). This was not observed in the contralateral hemispheres (a’, b’). Hemispheres treated with mZF-KRAB showed similar levels of Iba1+ cells compared with their contralateral non-injected hemispheres (c, c’, d, d’). Certain GFP-treated samples showed a slight increase in Iba1 immunoreactivity 4 weeks after treatment (e). Iba1 immunoreactivity was significantly increased 6 weeks after GFP injections, compared with the contralateral hemispheres (f, f’). PBS-injected samples show similar Iba1 immunoreactivity between hemispheres at both time points (g, g’, h, h’). Scale bar: 100 μmBack to article page