Fig. 6

Reduced αSyn pathology in IL-6 expressing mice at 2 months following intra-hippocampal injection of αSyn fibrils. Neonatal wild type mice were injected with rAAV-EV or rAAV-IL-6 and subsequently bilaterally injected in the hippocampus with αSyn fibrils at 2 months of age. pSer129 αSyn immunoreactive pathology was assessed in wild type mice 2 months post intra-hippocampal αSyn challenge. Immunolabeling with EP1536Y and 81A antibodies demonstrate reduced pathological αSyn levels in IL-6 mouse brains (a–b). Argyrophilic inclusions were also reduced in IL-6 cohort compared to the EV cohort (c). Accompanying panels on the right display the comparative distribution of pSer129 immunoreactive αSyn pathology on a representative brain map. The number of αSyn inclusions were counted in EP1536Y stained sections (whole brain) of IL-6 and EV cohorts injected with αSyn aggregates (d). *p < 0.05. n = 4–5 mice/cohort. Scale bar, 300 μm and 80 μm (inset)