Fig. 16

Lead decreases the total number of GAD67+ GABAergic interneurons in 20-week-old female ApoE4-KI mice. 8-week-old male and female ApoE3-KI and ApoE4-KI mice were exposed to 0.2% lead acetate for 12 weeks and sacrificed. a Representative images of GAD67 (red) immunostaining in the DG of 20-week-old female ApoE3-KI and ApoE4-KI control and lead-treated mice. b Quantification of the total number of GAD67+ cells in the DG of 20-week-old ApoE3-KI and ApoE4-KI females and males. Data are mean ± SEM with n = 3–5 per genotype/treatment. Two-way ANOVA with Fisher’s LSD post-test. Comparison between control and lead-treated: n.s., not significant; * p < 0.05. Comparison between males and females of the same genotype and treatment: n.s., not significant; #, p < 0.05; ##, p < 0.01. Scale bars, 100 μm