Fig. 5
From: Absence of CX3CR1 impairs the internalization of Tau by microglia
Tau phosphorylation, the number of microglial cells, colocalization between phosphorylated Tau and CX3CR1, and CX3CR1 expression increase in advanced stages of AD. Representative images of hippocampal tissue derived from AD patients showing microglia (Iba1, green), phospho-Tau (p-S396, white, red arrow) and DAPI (blue) staining at different stages of the disease: Braak-Tau I (a), Braak-Tau III (b) and Braak-Tau VI (c). Immunofluorescence images for Iba1- (green) and Tau-labeled structures (white, red arrow) are shown separately. d Representative high-power magnification image of microglia (green) and phospho-Tau (red) in Braak-Tau stage VI are shown. Orthogonal views are provided to highlight the colocalization between microglia and phospho-Tau. e Quantification of phospho-Tau fluorescence intensity present in the human hippocampus in AD. f Quantification of the number of microglia. g Colocalization between Iba1 and phospho-Tau at different stages of the disease. h Quantification of fluorescence intensity of CX3CR1 in the human hippocampus. (i-j) Representative images and quantification of the Cy5+ area relative to the internalization of Tau-Cy5 or phospho-Tau-Cy5 by microglia in vitro. Cell density was calculated as number of cells/μm3. Bars show means ± S.E. + p ≤ 0.05; ∗p ≤ 0.05; ∗∗ p ≤ 0.01 and ∗∗∗p ≤ 0.001. Red scale bar: 40 μm. White scale bar: 50 μm