Fig. 6

Mitochondria in rods with chronically active HIFs. a Pie chart of all identified and differentially regulated mitochondrial proteins in the photoreceptor segments (PS) of \(rod^{\varDelta \ Vhl}\) mice at 2.5 months of age. N = 6 mice per genotype. b Protein levels of the mitochondrial markers AIFM1, TOM70, and DLD in PS of \(rod^{\varDelta \ Vhl}\) mice and ctrl mice at 2.5 months of age. Shown are boxplots with the median and 25% and 75% percentiles of N = 6 mice per genotype. The samples with the highest (red) and lowest (blue) Cre levels are indicated. c Representative immunofluorescence labeling for VDAC1 (green, top panels) and COX4i1 (red, middle panels) in the PS region of \(rod^{\varDelta \ Vhl}\) and ctrl mice at the indicated time points. Bottom panels: merge. White arrows: regions with reduced COX4i1 labeling. N = 3 mice per genotype. d MT-CO2 levels in outer nuclear layer (ONL) and PS of \(rod^{\varDelta \ Vhl}\) and ctrl mice at 2.5 months of age. Shown are boxplots with the median and 25% and 75% percentiles of N = 6 mice per genotype. The samples with the highest (red) and lowest (blue) Cre levels are indicated. e Relative quantification of mtDNA copy number in \(rod^{\varDelta \ Vhl}\) and ctrl mice at 2.5 months of age. Shown are boxplots with the median and 25% and 75% percentiles of N = 6 mice per genotype. The samples with the highest (red) and lowest (blue) Cre levels are indicated. Statistics: Student’s t-test. f Representative immunofluorescence labeling for TFAM in the ONL and PS of \(rod^{\varDelta \ Vhl}\) and ctrl mice at time points as indicated. N = 3 mice per genotype. g Significantly regulated proteins involved in mtDNA maintenance (left), mtRNA metabolism (middle), and mitochondrial translation (right) in the PS and ONL of \(rod^{\varDelta \ Vhl}\) mice at 2.5 months of age. N = 6 mice per genotype. ND, not detected. For statistical analysis on proteomics data see Methods. *: p \(\le\) 0.05. OPL: outer plexiform layer. Scale bars, 50 \(\upmu\)m