Fig. 9

Experiments on ApoA1- and ApoE-immunodepleted CSF. A Protein aggregation assay performed using 0.7 mg/mL of recombinant α-syn in PBS with 40 μL of: CSF subjected to ApoA1 and ApoE IP (IP CSF), CSF subjected to IP without antibodies (IP CSF no Ab) and neat CSF belonging to different aliquots of the same NC pool. To remove the background fluorescence, the average fluorescence of three replicates containing the same reaction mix without α-syn was subtracted prior to the analysis. All ThT fluorescence traces are represented as average intensity over 3 replicates with error bars representing the SEM. B Average t2 fitted parameters with error bars representing the SEM. P-values were calculated by applying one-way ANOVA followed by Tukey post-hoc test