Fig. 2From: Amyloid-β (Aβ) immunotherapy induced microhemorrhages are associated with activated perivascular macrophages and peripheral monocyte recruitment in Alzheimer’s disease micePerivascular macrophages are highly associated with vascular amyloid in 3D6 treated PDAPP Mice. (a) Triple immunofluorescence of amyloid (Thio-S, green), microglia (P2Y12, red) and endothelial cells (PECAM-1, cyan) in PDAPP mice treated with 3D6 or IgG control. Thio-S, P2Y12 and PECAM-1 immunoreactivity overlay (Merge). Colocalization analysis (white). (b) Quantification of P2Y12+ area (%) of IgG or 3D6 treated mice. (c) Quantification of colocalization ratio of Thio-S and P2Y12. (d) Triple immunofluorescence of amyloid (Thio-S, green), macrophages (Mac387, red) and endothelial cells (PECAM-1, cyan) in PDAPP mice treated with 3D6 or IgG control. Thio-S, Mac387 and PECAM-1 immunoreactivity overlay (Merge). Colocalization analysis (white). (e) Quantification of Mac387+ area (%) of IgG or 3D6 treated mice. (f) Quantification of colocalization ratio of Thio-S and Mac387. (g) Triple immunofluorescence of amyloid (Thio-S, green), perivascular macrophages (CD169, red) and endothelial cells (PECAM-1, cyan) in PDAPP mice treated with 3D6 or IgG control. Thio-S, CD169 and PECAM-1 immunoreactivity overlay (Merge). Colocalization analysis (white). (h) Quantification of CD169+ area (%) of IgG or 3D6 treated mice. (i) Quantification of colocalization ratio of Thio-S and CD169. The number of vascular amyloid deposits analyzed was 8–10 per animal. Results are shown as ± SEM of n = 6 (mice). Asterisks indicate significant differences, where **p < 0.01, *** p < 0.001 or ****p < 0.0001 by unpaired Student’s t test. Scale bar 5 μm CC or 10 μm merge, respectivelyBack to article page