Fig. 1
Characterization of the microbead occlusion model and the ribotag mouse. A Schematic depicting the microbead occlusion model of elevating the IOP followed by IOP measurement. B IOP measures for the two cohorts of animals that were sacrificed at 7 and 30 days. N = 6 for each of the 3 independent groups for each 7 and 30 days; microbead injected mice, saline injected mice, and naïve untreated mice. Mean ± SEM. C Loss of ganglion cell density in the treated eye and loss of ganglion cell in the treated eye as a percentage of the untreated contralateral eye. N = 8 biological replicates. * p < 0.05. Paired students t-test. MB = microbead injected. D Immunostaining for HA and cell-specific markers in the optic nerve of RplHA/HA/Cre+ mice. All images are longitudinal sections of the nerve head region, with the retinal end of the optic nerve on the left. Scale bar in top left panel = 50 µm and applies to all panels. E RT-qPCR of the immunoprecipitate (IP) and input from 7 day microbead injected optic nerve heads showing enrichment of astrocyte ribosomal mRNAs over other cell types. N = 4 per group. Mean ± SEM