Fig. 6

Optic nerve head astrocytes show a limited neuroinflammatory transcriptional response. A RT-qPCR of the ONH input vs immunoprecipitate (IP) from untreated, 7 and 30 day microbead injected mice for select glaucoma-related neuroinflammatory genes. N = 4 per group. Mean ± SEM. * p < 0.05. B IOP measures over the 30 day experimental period. Same as in Fig. 1B, with the addition of IOP measurements from a cohort of mice that were injected with a larger concentration and volume of microbeads (High). N = 6 for each group. Mean ± SEM. * p < 0.01 (for the comparison between High vs Normal microbead injection). C Loss of ganglion cell density in the treated eye. Same as in Fig. 1C, with the addition of counts from a cohort of mice that were injected with a larger volume of microbeads (High). N = 8 biological replicates. * p < 0.05. Paired students t-test. D Loss of ganglion cells in the treated eye as a percentage of the untreated contralateral eye. Same as in Fig. 1C, with the addition of percentage loss from a cohort of mice that were injected with a larger volume of microbeads (High). N = 8 biological replicates. * p < 0.05. Paired students t-test. E RT-qPCR of the ONH immunoprecipitate (IP) from mice that were injected with the larger amount of microbead (High) vs those that were injected the normal amount (Normal) and tested for select glaucoma-related neuroinflammatory genes. N = 4 per group. Mean ± SEM. ns = not significant. Paired students t-test