Fig. 3
Mitovesicles isolated from the brain of Ts2 mice impair LTP. (A, B) Induced LTP (dashed lines) 20 min after hippocampal slices were perfused with either aCSF (Vehicle or Veh), or the indicated EV subtype (black arrow; 108 EVs/mL). Fr1 EVs (microvesicle-enriched, MicroV), Fr5 EVs (exosome-enriched, Exos), and Fr8 EVs (mitovesicle-enriched, mtVs), were chosen as the most representative fractions for each EV subtypes [2, 6,7,8] (see also Fig. 1). Ts2 mitovesicles impaired LTP, both when compared to 2N mitovesicles and to vehicle (A). In a separate set of experiments (B), microvesicles and exosomes (regardless of the genotype) had no effect on LTP. All tracks report means ± SEM. Two-way ANOVA for repeated measures was used for all comparisons. In (A): Vehicle (n = 16 slices from 13 mice) vs. Ts2 mitovesicles (n = 15 slices from 11 mice) P = 0.0022; 2N mitovesicles (n = 12 slices from 8 mice) vs. Ts2 mitovesicles P = 0.0290; 2N mitovesicles vs. vehicle P = 0.4422. In (B): 2N microvesicles (n = 10 slices from 7 mice) vs. vehicle (n = 12 slices from 7 mice) P = 0.7784; 2N exosomes (n = 13 slices from 8 mice) vs. vehicle P = 0.8164; 2N vs. Ts2 microvesicles (n = 12 slices from 5 mice) P = 0.5685; 2N vs. Ts2 exosomes (n = 10 slices from 5 mice) P = 0.7867; Ts2 mitovesicles (n = 10 slices from 7 mice) vs. vehicle P = 0.0275; Ts2 vs. 2N mitovesicles (n = 13 slices from 7 mice) P = 0.0419; Ts2 mitovesicles vs. Ts2 exosomes P = 0.0091; Ts2 mitovesicles vs. Ts2 microvesicles P = 0.0039. * P < 0.05, ** P < 0.01