Fig. 6
The LTP impairment induced by Ts2 mitovesicles is MAO-B-dependent. (A-C) Induced LTP (dashed lines) 20 min after hippocampal slices were perfused with either aCSF (Vehicle) or Fr8 EVs (mitovesicle-enriched, mtVs), as indicated in the figure. In (A), slices were treated or not with increasing doses of 2N mitovesicles. In (B), Ts2 mitovesicles were pretreated or not with PK (‘PK-shaving’) before addition to the hippocampal slices. In (C), mitovesicles were pretreated or not with 1 µM clorgiline (irreversible MAO-A inhibitor, MAO-Ai) or 1 µM pargyline (irreversible MAO-B inhibitor, MAO-Bi). LTP was restored when Ts2 mitovesicles were pretreated with MAO-Bi but not with MAO-Ai. All tracks report means ± SEM. Two-way ANOVA for repeated measures was used for all comparisons. In (A): Vehicle (n = 23 slices from 14 mice) vs. 108 (n = 14 slices from 9 mice) 2N mitovesicles P = 0.9342; Vehicle vs. 3 × 108 (n = 17 slices from 10 mice) 2N mitovesicles P = 0.0330; Vehicle vs. 10 × 108 (n = 13 slices from 8 mice) 2N mitovesicles P = 0.0423. In (B): Vehicle (n = 16 slices from 9 mice) vs. Ts2 mitovesicles (n = 11 slices from 5 mice) P = 0.0007; Ts2 mitovesicles vs. Ts2 mitovesicles + PK (n = 14 slices from 9 mice) P = 0.0021. In (C): Ts2 mitovesicles (n = 15 slices from 10 mice) vs. Ts2 mitovesicles + MAO-Bi (n = 17 slices from 10 mice) P = 0.0150; Ts2 mitovesicles vs. vehicle (n = 14 slices from 8 mice) P = 0.0158; Ts2 mitovesicles vs. Ts2 mitovesicles + MAO-Ai (n = 17 slices from 12 mice) P = 0.6630; vehicle vs. Ts2 mitovesicles + MAO-Ai: P = 0.0076. * P < 0.05, ** P < 0.01