Skip to main content
Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Regulation of human microglial gene expression and function via RNAase-H active antisense oligonucleotides in vivo in Alzheimer’s disease

Fig. 2

ASOs are internalized and pharmacologically active in human microglia xenografted in mouse brain. A ASO concentration in human and mouse microglia isolated from 10–12-week-old xenotrasplanted mice 7 days after administration of 125µg MALAT1 targeting ASO. B,C Expression of human MALAT1 (B) and mouse Malat1 (C) in isolated human and mouse microglia, respectively, from mice treated with MALAT1 ASO compared to vehicle treated mice. D Representative images of widespread ASO distribution 7 days post MALAT1 ASO treatment. Human microglia were identified as Iba1 and huNu positive cells, and mouse microglia as Iba1 positive and huNu negative cells. Nuclei were visualized with DAPI. White arrow indicates an example of human microglia, while yellow arrow indicates a mouse microglia. Scale bars represent 30µm. CNRQ = Calibrated Normalized Relative Quantities. Bars represent mean ± SEM, n = 5–7/group. Statistical differences based on the Mann–Whitney test: **p < 0.01

Back to article page

Molecular Neurodegeneration

ISSN: 1750-1326