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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Regulation of human microglial gene expression and function via RNAase-H active antisense oligonucleotides in vivo in Alzheimer’s disease

Fig. 3

(next page). Dose-dependent activity of APOE and TREM2 ASOs in human microglia xenografted in mouse brain. A,B Dose-dependent reduction in APOE (A) and TREM2 (B) expression in human microglia isolated from 10–12-week-old xenotransplanted mice 7 days after administration of APOE ASO-1 (A) or TREM2 ASO-171 (B) (red dots/line-knockdown level, blue dots/line-ASO concentration). (C,D) Expression of mouse ApoE (C) and Trem2 (D) in isolated mouse microglia after treatment with 45µg APOE ASO-1 (C) or 90µg TREM2 ASO-171 (D). CNRQ = Calibrated Normalized Relative Quantities Dots or bars represent mean ± SEM, n = 2–10/group. E–F ASO concentrations in human and mouse microglia isolated from 10–12-week-old xenotransplanted mice 7 days after administration of APOE ASO-1 (E) or TREM2 ASO-171 (F). Mean + SD, n = 3

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Molecular Neurodegeneration

ISSN: 1750-1326