Ginkgolides protect against amyloid-β1–42-mediated synapse damage in vitro
© Bate et al; licensee BioMed Central Ltd. 2008
Received: 14 October 2007
Accepted: 07 January 2008
Published: 07 January 2008
The early stages of Alzheimer's disease (AD) are closely associated with the production of the Aβ1–42 peptide, loss of synapses and gradual cognitive decline. Since some epidemiological studies showed that EGb 761, an extract from the leaves of the Ginkgo biloba tree, had a beneficial effect on mild forms of AD, the effects of some of the major components of the EGb 761 extract (ginkgolides A and B, myricetin and quercetin) on synapse damage in response to Aβ1–42 were examined.
The addition of Aβ1–42 to cortical or hippocampal neurons reduced the amounts of cell associated synaptophysin, a pre-synaptic membrane protein that is essential for neurotransmission, indicating synapse damage. The effects of Aβ1–42 on synapses were apparent at concentrations approximately 100 fold less than that required to kill neurons; the synaptophysin content of neuronal cultures was reduced by 50% by 50 nM Aβ1–42. Pre-treatment of cortical or hippocampal neuronal cultures with ginkgolides A or B, but not with myrecitin or quercetin, protected against Aβ1–42-induced loss of synaptophysin. This protective effect was achieved with nanomolar concentrations of ginkgolides. Previous studies indicated that the ginkgolides are platelet-activating factor (PAF) receptor antagonists and here we show that Aβ1–42-induced loss of synaptophysin from neuronal cultures was also reduced by pre-treatment with other PAF antagonists (Hexa-PAF and CV6209). PAF, but not lyso-PAF, mimicked the effects Aβ1–42 and caused a dose-dependent reduction in the synaptophysin content of neurons. This effect of PAF was greatly reduced by pre-treatment with ginkgolide B. In contrast, ginkgolide B did not affect the loss of synaptophysin in neurons incubated with prostaglandin E2.
Pre-treatment with ginkgolides A or B protects neurons against Aβ1–42-induced synapse damage. These ginkgolides also reduced the effects of PAF, but not those of prostaglandin E2, on the synaptophysin content of neuronal cultures, results consistent with prior reports that ginkgolides act as PAF receptor antagonists. Such observations suggest that the ginkgolides are active components of Ginkgo biloba preparations and may protect against the synapse damage and the cognitive loss seen during the early stages of AD.
Alzheimer's disease (AD) is a complex and genetically heterogeneous disease that is the most common form of dementia and affects up to 15 million individuals worldwide. The amyloid hypothesis of AD pathogenesis maintains that the primary event is the production and accumulation of amyloid-β (Aβ) peptides, derived from abnormal proetolytic cleavage of the amyloid precursor protein [1–3]. The accumulation of Aβ peptides leads to the subsequent disruption of neuronal processes, abnormal phosphorylation of tau and ultimately the dysfunction and death of neurons. However, the precise mechanisms by which Aβ peptides lead to neuronal damage remain to be fully determined. Initially it was thought that fibril formation by Aβ peptides was required for neurotoxicity , however, more recent studies showed that smaller soluble oligomers of Aβ or Aβ-derived diffusible ligands are also potent neurotoxins [5, 6].
The early stages of AD are characterised by memory impairment and subtle behavioural changes, associated with changes in synaptic function and a reduction in the levels of synaptophysin, a presynaptic membrane protein essential for neurotransmitter release and the recycling of synaptic vesicles , within the brain. These occur before any gross neurological damage is observed [8–10]. The loss of synapses and the reduction in synaptophysin levels are features of AD that strongly correlate with cognitive decline . We previously developed an in vitro model to examine the effects of Aβ peptides on synapses where the amounts of synaptophysin in neuronal cultures were measured as a surrogate marker of synapse function. The addition of Aβ1–42 reduced the synaptophysin content of neurons indicating the loss of synapses in these cultures . In this paper, a possible mechanism leading to Aβ1–42-induced loss of synaptophysin from neuronal cultures was investigated.
Extracts from the leaves of the Ginkgo biloba tree are becoming increasingly popular as a treatment that is claimed to reduce memory loss and the symptoms of mild cognitive disorders including AD [13–15]. However, there remains considerable controversy regarding the mechanisms of action of these preparations, or even whether such preparations have any clinical benefit. While some published studies conclude that the use of a standardized extract of the leaves of the Ginkgo biloba tree (EGb 761) reduces the symptoms of mild cognitive disorders including AD [13, 16], other studies have failed to show clinical benefit . Since the EGb 761 extract contains many compounds, including ginkgolides and the flavonoglycosides myricetin and quercetin, it is not clear which individual components of this extract are efficacious. Our previous studies showed that pre-treatment with ginkgolides protected against Aβ1–42-induced neuronal death  and in the current study we tested the main compounds in the EGb 761 extract on cultured neurons and for their effects on synapse damage in response to Aβ1–42. We report that pre-treatment of cultured neurons with ginkgolides A or B significantly reduced the effects of Aβ1–42 on synapses.
Ginkgolides protect against Aβ1–42-induced synapse damage
Ginkgolides do not affect the binding of Aβ1–42to neurons
PAF mimics the effects of Aβ1–42on synaptophysin levels
Ginkgolide B does not protect against PGE2-induced synapse damage
Ginkgolide B does not affect PGE2-induced loss of synaptophysin.
Synaptophysin content (units)
Ginkgolide B (1 μM)
AH13205 (100 nM)
100 ± 5
101 ± 3
102 ± 3
Aβ 1–42 (200 nM)
24 ± 4
93 ± 7
97 ± 6
PAF (10 nM)
33 ± 7
78 ± 5
89 ± 8
PGE 2 (40 nM)
41 ± 6
44 ± 7
96 ± 4
Butaprost (1 μM)
29 ± 5
37 ± 11
94 ± 7
Misprostal (1 μM)
32 ± 7
34 ± 6
98 ± 5
An in vitro model of Aβ-mediated synapse damage was used to investigate the effects of individual components of the Gingko biloba extract EGb 761 on the synapse damage that occurs in AD. The addition of Aβ1–42, and to a lesser extent Aβ1–40, reduced the synaptophysin content of cortical and hippocampal neuronal cultures. The hippocampus is thought to be involved in the formation of short term memory, the loss of which is one of the earliest clinical symptoms of AD . In all assays, the effects of Aβ peptides on the synaptophysin content of neuronal cultures occurred at concentrations significantly lower than those required to kill neurons. These observation are consistent with in vivo observations that show that a loss of synapses and a reduction in synaptophysin levels occurs before any gross neurological damage is observed [8–11].
Although previous studies suggest that the flavonoglycosides have protective properties against oxidative stress in vitro [14, 21], no protective effects of myricetin or quercetin against Aβ1–42-induced synapse damage were observed in these studies. Moreover, flavonoglycosides have limited bioavailability after oral administration  suggesting that these compounds are less likely to be responsible for the protective effects of the EGb 761 extract. In contrast, nanomolar concentrations of both ginkgolides A and B reduced the loss of synaptophysin induced by Aβ1–42 in cortical neuronal cultures. Pre-treatment with 1 μM ginkgolide B increased the amounts of Aβ1–42 required to reduce the synaptophysin content of cortical neurons by 50% from 50 nM in untreated neurons to 8 μM in ginkgolide B-treated neurons. The addition of Aβ1–42 also damaged synapses in cultured hippocampal neurons; it required only 10 nM Aβ1–42 to reduce the synaptophysin content of these cultures by 50%. While it is tempting to suggest that hippocampal neuronal synapses are more sensitive to Aβ1–42 than cortical neuronal synapses a direct comparison is invalid considering the differences in the ages of mice used and the number of contaminating astroglial cells. In hippocampal neuronal cultures pre-treated with ginkgolide B over 1 μM Aβ1–42 was needed to reduce the synaptophysin content by 50%.
The mechanism of the protective effect of ginkgolides was sought. We dismissed the simplest explanation, that ginkgolides reduce the ability of neurons to bind Aβ peptides, because ginkgolide-treated neurons ingested similar amounts of biotinylated-Aβ1–42 as untreated neurons. Aβ1–42 peptides exist in different states ranging from small soluble oligomers or Aβ-derived diffusible ligands [5, 6], to larger fibrillar structures . Therefore it is possible that ginkgolides bind directly to Aβ1–42 peptides and promote the formation of an inactive conformer. Although this hypothesis cannot be totally excluded, ginkgolide-treated cultures that had been washed (which removed any non-bound ginkgolides) remained resistant to the synapse damage induced by Aβ1–42. We note with interest an observation that Aβ fibrillogenesis is accelerated in the presence of plasma, endosomal and lysosomal membranes, but reduced in Golgi membranes. The authors suggested that the composition of these membranes affected Aβ oligomerisation  and the possibility that ginkgolides affect membrane composition and subsequently Aβ fibril formation within intracellular compartments cannot be excluded.
Since Aβ peptides activate phospholipase A2 (PLA2) , a major step in the production of PAF , the possibility that synapse damage occurs as a consequence of Aβ1–42-induced production of PAF was investigated. PAF receptors are present in synapses  and PAF is required for synapse maintenance  and long term potential in the hippocampus . However, higher concentrations of PAF are implicated in neurodegenerative diseases including AD . Here we report that the addition of PAF caused a dose-dependent reduction in the synaptophysin content of cortical neurons; 5 nM PAF reduced synaptophysin content by 50%. The effects of PAF are transmitted via a G-protein coupled receptor  and the addition of lyso-PAF, a non-acetylated structural analogue of PAF that does not bind to the PAF receptor, did not cause synapse damage. One of the many reported effects of the ginkgolides is antagonism of PAF receptors  and in the current study we demonstrated that ginkgolide-treated neurons were partially resistant to the effects of PAF, as well as that of Aβ1–42, on synapses. The synapse-protective effect of ginkgolide B was consistently stronger than ginkgolide A, consistent with reports that ginkgolide B has greater affinity for PAF receptors than ginkgolide A . Pre-treatment with two non-related PAF antagonists, hexa-PAF and CV6209 [31, 32], also protected synapses suggesting that antagonism of the PAF receptors mediates the synaptoprotective effects of the ginkgolides. Although pre-treatment with a combination of ginkgolide B and hexa-PAF increased the synaptophysin content of cultures relative to treatment with ginkgolide B or hexa-PAF alone, their affects appeared to be additive rather than synergistic.
Both Aβ1–42 and PAF, but not lyso-PAF, increased the production of PGE2 in cortical neurons in vitro  raising the possibility that the effects of PAF on synapses were mediated through the production of PGE2. While pre-treatment with ginkgolide B reduced both Aβ1–42 and PAF-induced production of PGE2, it did not alter the effects of PGE2, or two EP receptor agonists (butaprost or misoprostol), on the synaptophysin content of neuronal cultures. In contrast, the EP receptor antagonist AH13205  protected against Aβ1–42, PAF and PGE2-induced synaptophysin loss. These results are consistent with the hypothesis that ginkgolides protect against Aβ1–42-mediated synapse damage by modifying the production of PGE2. This hypothesis is also consistent with epidemiological data that show that the use of cyclo-oxygenase inhibitors reduce the severity of AD . We suggest that activation of PLA2 by Aβ1–42 leads to sustained production of PAF which in turn increases PGE2 production, and that high concentrations of PGE2 damaged synapses. PGE2 modulates synaptic transmission  and although high concentrations were reported to induce apoptosis in hippocampal neurons , we found that 10 μM PGE2 did not kill cortical neurons (data not shown). Similarly we found that although the EP receptor antagonist AH13205 reduced Aβ1–42-induced loss of synaptophysin it did not affect Aβ1–42-induced neuronal death (data not shown).
It is worth noting that for many Ginkgo biloba extracts, extraction procedures are used that optimise the flavonoglycoside content. However, these procedures may result in extracts that contain different amounts of ginkgolides, and a recent analysis of commercial Ginkgo biloba supplements by liquid chromatography/mass spectrometry demonstrated remarkable variations in ginkgolide content . Therefore, variations in the ginkgolide content of different extracts may be a factor that explains the variability of results obtained in clinical studies.
In summary we show that sub-toxic concentrations of Aβ1–42 damaged synapses in cultured cortical and hippocampal neurons. We propose that these changes model the early stages of AD, where there are significant behavioural changes including synaptic dysfunction and alterations in the development of short-term memory, but before gross neuronal loss is observed . We report that neurons treated with ginkgolides A or B show increased resistance to the effects Aβ1–42 on synapses. This effect of the ginkgolides correlated with their ability to antagonise PAF receptors , and in these studies PAF also reduced the synaptophysin content of cortical neuronal cultures. Recent studies showed that the bioavailability of ginkgolides after oral administration is high  suggesting that the ginkgolides have the potential to reduce synapse damage in AD should they be able to cross the blood brain barrier and penetrate the central nervous system.
Cortical neurons were prepared from the brains of day 15.5 C57BL/6J mouse embryos. After mechanical dissociation, cell sieving and isolation on histopaque (Sigma, Poole, UK), neuronal precursors were plated at 2 × 105 cells/well in 48 well plates in Hams F12 containing 5% foetal calf serum (FCS) for 2 hours. Cultures were then shaken (600 r.p.m for 5 minutes) and non-adherent cells removed by 2 washes in phosphate buffered saline (PBS). Neurons were grown in neurobasal medium (NBM) containing B27 components (Invitrogen, Paisley, UK) for 7 days. Immunostaining showed that the cells were greater than 97% neurofilament positive. Less than 3% stained for GFAP (astrocytes) or for F4/80 or CD14 (microglial cells).
Hippocampal neuronal cultures were prepared from the brains of adult C57BL/6J mice as previously described . Briefly, hippocampi were dissected from the adult brain tissue and triturated with fire-polished pasteur pipette in Hams F12 containing 5% FCS, 0.35% glucose, 0.025% trypsin, and 0.1% type IV collagenase (Invitrogen). After 30-minute incubation at 37°C, the cells were shaken and the cell suspension was passed through a 100 μM cell strainer (Becton Dickenson). Cells were collected by centrifugation, washed twice in Hams F12 containing 5% FCS, and plated at 2 × 105 cells/well in 48 well plates for 24 hours. Cultures were shaken (600 r.p.m for 5 minutes) to remove non-adherent cells, washed twice with PBS and the remaining cells were cultured in NBM containing B27 components and 10 ng/ml glial-derived neurotrophic factor (Sigma) for 7 days. Immunostaining showed that after 7 days cultured in NBM less than 5% of the viable cells stained for GFAP or F4/80 (astrocytes or microglial cells). Both types of neuronal cultures were pre-treated with test compounds for 3 hours. Cells were then washed with PBS 3 times to remove unbound test compounds before the addition of Aβ peptides in NBM/B27 components; the amounts of synaptophysin in treated cultures were measured 24 hours later.
Treated neurons were washed 3 times with PBS and homogenised in a buffer containing 150 mM NaCl, 10 mM Tris-HCl, 10 mM EDTA, 0.2% SDS and mixed protease inhibitors (AEBSF, Aprotinin, Leupeptin, Bestain, Pepstatin A and E-46) (Sigma) at 1 × 106 cells/ml. Nuclei and cell debris was removed by centrifugation (300 × g for 5 minutes).
Synaptophysin levels in neuronal extracts were measured by a sandwich ELISA as previously described . In short, the capture antibody (0.5 μg/ml) was a mouse monoclonal antibody (mab) anti-synaptophysin MAB368 (Chemicon, UK) and bound synaptophysin was detected using a goat polyclonal anti-synaptophysin (Abcam, Chandler's Ford, UK). Bound antibodies were detected with a biotinylated anti-goat IgG (Dako, Cambridgeshire, UK), extravidin-alkaline phosphatase and 1 mg/ml 4-nitrophenol phosphate in diethanolamine buffer (Sigma). Absorbance was measured on a microplate reader at 450 nM and the synaptophysin content was calculated by reference to a standard curve. Samples were expressed as "units synaptophysin" where 100 units was arbitrarily defined as the amount of synaptophysin in 1 × 106 untreated cells. A standard curve was generated from this sample using sequential log 2 dilutions (range 100 to 1.56 units).
Ingestion of biotinylated-Aβ1–42
Cortical neurons were pre-treated with drugs for 3 hours and washed 3 times with PBS prior to the addition of biotinylated-Aβ1–42. After 10, 30 or 60 minutes neurons were washed a further three times with warm PBS to remove unbound peptide and then homogenised in a buffer containing 150 mM NaCl, 10 mM Tris-HCl, 10 mM EDTA, 0.2% SDS and mixed protease inhibitors at 1 × 106 cells/ml. Nuclei and cell debris was removed by low speed centrifugation (300 × g for 5 minutes). The amounts of biotinylated-Aβ1–42 in cell extracts were determined by ELISA. All stages of the ELISA were carried out at 37°C for 1 hr. Maxisorb Immunoplates (Nunc, Roskilde, Denmark) were coated with 0.1 μg mouse mab to Aβ1–42 (Immunodiagnostic Systems, Tyne and Wear, UK) as a capture antibody. Samples were applied and the amounts of biotinylated-Aβ1–42 bound were determined by incubation with extravidin-alkaline phosphatase and 1 mg/ml 4-nitrophenol phosphate in diethanolamine buffer. Absorbance was measured at 450 nM and the amounts of biotinylated-Aβ1–42 in samples were expressed as optical density (OD) × 1000.
A peptide corresponding to amino acids 1 to 42 of the Aβ protein (Aβ1–42), a biotinylated version containing biotin at the terminal aspartic acid residue (biotinylated-Aβ1–42), Aβ1–40 and a control peptide (Aβ42-1) were obtained from Bachem (St Helens, UK). 1 mM stock solutions of peptide were kept at -70°C, thawed on the day of use and sonicated prior to dilution and addition to cells.
CV6209, ginkgolide B, myricetin, quercetin, platelet-activating factor (PAF), lyso-PAF, butaprost, misoprostol and AH13025 were obtained from Sigma. Ginkgolide A, hexa-PAF and PGE2, were obtained from Calbiochem (Nottingham, UK).
Comparison of treatment effects were carried out using one and two way analysis of variance techniques as appropriate. Post hoc comparisons of means were performed as necessary.
- (EGb 761):
a standardized extract of the leaves of the Ginkgo biloba tree
fetal calf serum
This work was supported by a grant from the European Commission FP6 "Neuroprion" – Network of Excellence.
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