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Table 1 Peptides and MRM transitions used for the quantification of clusterin

From: Mass spectrometry quantification of clusterin in the human brain

 

MRM transitions (m/z)

Peptides

Precursor

 

Product

 

ASSIIDELFQDR (Q1 ,light)

697.35 (+2)

678.40 (y5,+1)

922.43 (y7,+1)

1035.51 (y8,+1)

ASSIIDELFQDR (Q1 ,heavy)

702.36 (+2)

688.40 (y5,+1)

932.43 (y7,+1)

1045.52 (y8,+1)

ELDESLQVAER (Q4 ,light)

644.82 (+2)

602.30 (y5,+1)

802.44 (y7,+1)

1046.51 (y9,+1)

ELDESLQVAER (Q4 ,heavy)

649.83 (+2)

612.30 (y5,+1)

812.45 (y7,+1)

1056.52 (y9,+1)

VTTVASHTSDSDVPSGVTEVVVK (Q5 ,light)

772.06 (+3)

507.80 (y10,+2)

773.48 (y7,+1)

1014.58 (y10,+1)

VTTVASHTSDSDVPSGVTEVVVK (Q5 ,heavy)

774.74 (+3)

511.80 (y10,+2)

781.49 (y7,+1)

1022.60 (y10,+1)

  1. [13C6,15 N2]-Lys and [13C6,15 N4]-Arg in labeled peptides are shown in bold. For MRM transitions, charge for precursor ions, and, and the type and charge for product ions are shown in parentheses.