Figure 4
From: Heteromers of amyloid precursor protein in cerebrospinal fluid
sAPPf immunoreactivity levels in CSF from NDC and AD subjects. Immunodetection of sAPPf in CSF samples from 13 NDC and 13 AD subjects. (A) Representative blot of CSF-sAPPf resolved with the Covance-Ct antibody. The 130 kDa band was not easily visualized in all samples, and was quantified at longer exposure than the 100 kDa band. (B) Densitometric quantification of the APP-immunoreactive bands, ~110 and 130 kDa, from the NDC and AD cases (performed in duplicate). A control CSF sample was run on different gels (processed in parallel) to normalize the immunoreactive signal between immunoblots. Differences were found only for the 130 kDa band between NDC and AD subjects. The data represent the means ± SEM (n.s., not significant).