Figure 2From: Ubiquitin-specific protease 14 regulates c-Jun N-terminal kinase signaling at the neuromuscular junction Neuronal expression of USP14CA leads to decreased body weight, muscle weight, and motor performance. (A) Survival curves for wild type, TgUsp14CA, TgUsp14, and ax J mice. TgUsp14 mice express wild type USP14 under the Thy1.2 promoter and serve as a control for neuronal USP14 overexpression in TgUsp14CA mice. ax J mice have a 95% reduction of USP14 in all tissues. (B) Tail suspension assays show hind limb clasping in TgUsp14CA mice versus the typical splaying observed in 6-week-old wild type and TgUsp14 mice. Assays of (C) body mass, (D) muscle mass, (E) muscle strength and (F) motor performance of 2- to 24-week-old wild type, TgUsp14CA, and TgUsp14 mice. Muscle strength was assessed by a forelimb grip-force assay and motor performance was assessed by time required to traverse an elevated beam. All data are shown as mean ± SEM. Symbols represent Mann-Whitney tests compared against wild type and corrected for multiple comparisons with a Bonferonni adjustment where appropriate; *p < 0.05, **p < 0.01, ***p < 0.001; n = at least 4 animals per genotype per time point.Back to article page