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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Ubiquitin-specific protease 14 regulates c-Jun N-terminal kinase signaling at the neuromuscular junction

Figure 5

Loss of USP14’s ubiquitin hydrolase activity results in accumulation of non-proteasomal associated ubiquitin conjugates. (A) Representative immunoblot of spinal cord extracts from 4- to 6-week-old wild type and TgUsp14CA mice and cortical neurons treated with vehicle or 20 μM IU1 for 24 h and probed with an antibody specific for K48-linked ubiquitin chains. (B) Quantitation of (A). (C) As in (A) except with anti-K63-linked ubiquitin chain antibody. β-tubulin was used as a loading control in all experiments. (D) Quantitation of (D). Data are shown as mean ± SEM, and n = at least 3 animals per genotype or condition run in duplicate. Symbols represent Mann-Whitney tests, where *p < 0.05 and **p < 0.01. See also Additional file 3.

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Molecular Neurodegeneration

ISSN: 1750-1326