Figure 6
Effects of piceid and L-dopa on MPTP-induced PD in mice. Locomotor activity (A) and rotarod activity (B) on treated and untreated C57BL/6 mice determined on the 15th day (N = 10 animals each troup). C) The actual SOD activity and MDA levels. “* and **” indicate P < 0.05 and 0.01, respectively, as compared to the MPTP-induced model group. L50 and L25 indicate L-Dopa at 50 and 25 mg/kg dosages, respectively. D) Immunohistochemistry. Coronal sections of paraffin blots (6 μcrom) were stained with H & E, mouse anti-TH (both DAB and fluorescent readouts) and antibodies against p-Akt (Ser374) and activated caspase-3. Representative images were chosen from a set of three mice each group. E) Western blot analysis of the isolated SNc tissue (lysates pooled from 3 mice each group). Trx: thioredoxin; F) Quantification. “***” indicates P < 0.005 as compared to the MPTP group.