Figure 4

Over-expression of individual secretase genes in SY5Y-APP-Gal4 cells increases AICD-Gal4 mediated luciferase activity. (A) Transient over-expression of ADAM10 increases ADAM10, AICD-Gal4, C83-Gal4, and sAPPα levels compared to cells transfected with empty vector. (B) Quantification of Western blot densitometry in panel A. (C) ADAM17 transient over-expression significantly increases ADAM17, AICD-Gal4, C83-Gal4, and sAPPα levels. (D) Quantification of Western blot densitometry in panel C. (E) Transient over-expression of individual secretase genes increases AICD-Gal4 mediated luciferase activity. Luciferase was normalized to transfection efficiency, by dividing by Renilla luciferase activity. Individual secretase over-expression plasmids were co-transfected with pRL-SV40 plasmid, expressing Renilla luciferase. Bars represent the mean normalized luciferase activity of four independent trials and error bars represent standard errors. Statistical significance was determined using two-sample, one-tail t-tests to compare each secretase gene with the empty vector, followed by sequential Bonferroni procedure to adjust for multiple comparisons. * indicates p < 0.05; ** indicates p < 0.01.