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Table 1 Experimental design of the neuroprotection and regeneration study

From: Dopaminergic regeneration by neurturin-overexpressing c17.2 neural stem cells in a rat model of Parkinson's disease

Protection Study

NTN (n = 15)

Mock (n = 15)

PBS (n = 15)

15 days

6-OHDA lesion

 

4.5 days or every month

(n = 15 in each group)

Rotation behavior and Sacrifice for IHC, HPLC

Regeneration Study

6-OHDA lesion (n = 45)

4 weeks

Rotation Behavior

NTN (n = 15)

Mock (n = 15)

PBS (n = 15)

every month

(n = 15 in each group)

Rotation behavior and Sacrifice for IHC, HPLC

  1. NTN-c17.2, Mock- c17.2, and PBS were transplanted into the striatum by the conventional microinjection method. Subsequently, 6-hydroxydopamine (6-OHDA) was infused into the striatum or into the medial forebrain bundle (MFB) and the ventral tegmental area (VTA) in the neuroprotection study or regeneration study, respectively (see Materials and Methods). After transplantation, all the animals were analyzed for apomorphine-induced rotational behavior and then sacrificed for immunohistochemistry (IHC) and high-performance liquid chromatography (HPLC) measurements.
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Molecular Neurodegeneration

ISSN: 1750-1326