APP-BP1 and PS1 molecules were colocalized in primary neurons. Primary neurons were fixed, permeablized, blocked and stained with 9E10 for mycAPP-BP1 and the rabbit anti-PS1 (AB5308). Primary antibodies were coupled to Alexa Fluor 594 and Alexa Fluor 488 goat secondary antibodies. The images presented were obtained under a 40× objective with a Nikon confocal microscope using the EZ-C12.20 scanning program. Independent experiments using DAPI staining along with the above two antibodies did not show nuclear co-localization of APP-BP1 and PS1 (not shown).