AIDtg expression in cultured fetal primary neurons does not change the relative expression of APP, NEP, KAI1 and p53. A AIDtg expression was confirmed by tail genotyping of fetuses (not shown) and by QPCR data on cultured neurons (dark bars). Expression of AID, A, APP, B, NEP, C, KAI1, D, and p53, E, is relative to the 100% value given arbitrarily to the first AIDtg mouse. Experiments were conducted in triplicate loading, and the error bars represent standard deviations. Cultures were harvested at DIV 14. Similar results were achieved from younger cultures (DIV 9, not shown) and in the AIDTg 59 line (not shown).