Figure 2

Ectopic localization of S-phase nuclei in the ventricular zone of PS cDKO brains. (A, B) At E13.5, in control brains, the BrdU-positive nuclei are localized in the upper ventricular zone closer to the preplate, whereas in PS cDKO brains, they are distributed more broadly and predominantly in the lower ventricular zone closer to the ventricle, suggesting disrupted interkinetic nuclear migration. (C, D) At E14.5, in PS cDKO brains, the BrdU-positive nuclei are absent in many areas of the ventricular zone and are distributed close to the lateral ventricle. (E) The ventricular zone that contains BrdU-positive cells is divided by 11 bins (15 μm/bin). Bin 1 is the layer closest to the preplate (PP), whereas bin 11 is the layer closest to the lateral ventricle (LV). n = 5 for the controls and n = 6 for PS cDKO embryos. Scale bar: 100 μm in all figures.